منابع مشابه
Production of prone-to-aggregate proteins.
Expression of recombinant proteins in Escherichia coli (E. coli) remains the most popular and cost-effective method for producing proteins in basic research and for pharmaceutical applications. Despite accumulating experience and methodologies developed over the years, production of recombinant proteins prone to aggregate in E. coli-based systems poses a major challenge in most research applica...
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Many neurodegenerative diseases are caused by intracellular, aggregate-prone proteins, including polyglutamine-expanded huntingtin in Huntington's disease (HD) and mutant tau in fronto-temporal dementia/tauopathy. Previously, we showed that rapamycin, an autophagy inducer, enhances mutant huntingtin fragment clearance and attenuated toxicity. Here we show much wider applications for this approa...
متن کاملLithium rescues toxicity of aggregate-prone proteins in Drosophila by perturbing Wnt pathway.
We have previously shown that lithium can protect against the polyglutamine toxicity of the Huntington's disease mutation in cell models. Here, we demonstrate for the first time in vivo that lithium can protect against the toxicity caused by aggregate-prone proteins with either polyglutamine or polyalanine expansions in Drosophila. We also show that these protective effects can be partly accoun...
متن کاملAggregate-prone proteins with polyglutamine and polyalanine expansions are degraded by autophagy.
Protein conformational disorders (PCDs), such as Alzheimer's disease, Huntington's disease (HD), Parkinson's disease and oculopharyngeal muscular dystrophy, are associated with proteins that misfold and aggregate. Here we have used exon 1 of the HD gene with expanded polyglutamine [poly(Q)] repeats and enhanced green fluorescent protein tagged to 19 alanines as models for aggregate-prone protei...
متن کاملApplying Differential Evolution to Aggregate Production Planning
Differential evolution (DE) has become a popular and promising technique in the recent decade. There are many successful applications implemented DE in scientific and engineering fields. However, we rarely found it applying to the field of highly constrained aggregate production planning (APP) problems. Besides, in the course of solving the APP problem, we discover that the most often used DE s...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 2013
ISSN: 0014-5793
DOI: 10.1016/j.febslet.2013.10.044